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目的:为寻找中药重楼的替代资源,研究巴山重楼根茎的化学成分。方法:依次用正相硅胶柱色谱、Sephadex LH-20凝胶柱色谱、反相硅胶柱色谱、反相制备HPLC等手段,从巴山重楼根茎的正丁醇提取部分分离纯化单体化合物,并运用MS、1H-NMR、13C-NMR、2D-NMR等波谱技术鉴定所得化合物的结构。结果:从巴山重楼的正丁醇提取部分中分离得到5个化合物,分别鉴定为:β-蜕皮激素(1)、pinnatasterone(2)、偏诺皂苷元-3-O-α-L-鼠李吡喃糖(1→2)-[α-L-阿拉伯呋喃糖(1→4)]-β-D-葡萄吡喃糖苷(3)、薯蓣皂苷元-3-O-α-L-鼠李吡喃糖(1→2)-[α-L-阿拉伯呋喃糖(1→4)]-β-D-葡萄吡喃糖苷(4)、偏诺皂苷元-3-O-α-L-鼠李吡喃糖(1→4)-α-L-鼠李吡喃糖(1→4)-[α-L-鼠李吡喃糖(1→2)]-β-D-葡萄吡喃糖苷(5)。结论:化合物1～5为首次在该植物中分离得到。
Objective: In order to find alternative resources of re-building of Chinese medicine, the chemical composition of rhizome of Bashan re-building was studied. Methods: The compounds were separated and purified from the n-butanol extracts of Rhizoma Parus communis by normal phase silica gel column chromatography, Sephadex LH-20 column chromatography, reverse phase silica gel column chromatography and reversed phase preparative HPLC. The structures of the obtained compounds were identified by MS, 1H-NMR, 13C-NMR, 2D-NMR and other spectroscopic techniques. Results: Five compounds were isolated from the n-butanol fraction of Basiletia soongorica and identified as β-ecdysone (1), pinnatasterone (2), sapogenin-3-O-α-L- Pyranose (1 → 2) - [α-L-arabinofuranose (1 → 4)] - β-D-glucopyranoside (3), diosgenin-3-O-α-L- Pyranosyl (1 → 2) - [α-L-arabinofuranosyl (1 → 4)] - β-D-glucopyranoside (4) (1 → 4) -α-L-rhamnopyranose (1 → 4) - [α-L-rhamnopyranose (1 → 2)] - β-D-grape pyran Glycoside (5). Conclusion: Compounds 1 ~ 5 were isolated from this plant for the first time.