在酸性介质中,双酚A对羟基自由基氧化结晶紫的反应具有抑制作用,据此建立了分光光度法测定痕量双酚A的新方法。在最佳实验条件下测量结晶紫580nm处的吸光度变化值,得到方法的线性范围是0.015—0.25μg/mL,相关系数为0.9994,检出限和定量下限分别为3.3、11.0ng/mL。应用此法测定了6种样品中双酚A的含量,回收率在91%—118%之间,相对标准偏差为1.3%—6.8%。该方法简便快速,结果稳定准确。 In acid medium, the reaction of bisphenol A with hydroxy radical on crystal violet has an inhibitory effect, and a new method of spectrophotometric determination of trace bisphenol A has been established. Under the optimal experimental conditions, the change of absorbance at crystal violet 580nm was measured. The linear range of the method was 0.015-0.25μg / mL with the correlation coefficient of 0.9994. The detection limits and the limits of quantification were 3.3 and 11.0ng / mL, respectively. The contents of bisphenol A in six samples were determined by this method. The recoveries were between 91% and 118% with relative standard deviations of 1.3% -6.8%. The method is simple and fast, the results are stable and accurate.