【摘 要】
：
目的:探讨大鼠口腔癌变过程中线粒体琥珀酸脱氢酶(Succinate dehydrogenase,SDH)的活性变化及其生物学意义。方法:2×10-5 4-硝基喹啉-1-氧化物(4NQO)饮水喂养Wistar大鼠9~32
【机 构】
：
上海交通大学医学院附属第九人民医院口腔黏膜科·口腔医学院·上海市口腔研究所·上海市口腔医学重点实验室,上海交通大学附属第一人民医院口腔科,
论文部分内容阅读
目的:探讨大鼠口腔癌变过程中线粒体琥珀酸脱氢酶(Succinate dehydrogenase,SDH)的活性变化及其生物学意义。方法:2×10-5 4-硝基喹啉-1-氧化物(4NQO)饮水喂养Wistar大鼠9~32周建立大鼠舌癌变模型,提取30例大鼠舌癌变过程不同病理阶段的舌背组织线粒体,酶标仪检测SDH酶活性的变化。结果:在大鼠舌白斑癌变过程中,线粒体SDH活性呈逐渐下降趋势,其中,重度异常增生、鳞癌组织中SDH酶活性显著低于正常黏膜(P<0.05)。SDH的基因表达及酶活性变化趋势较为一致。结论:SDH酶活性和m RNA表达水平的下降与口腔癌发生发展相关,三羧酸循环在口腔黏膜癌变过程可能起到重要作用。
Objective: To investigate the changes of mitochondrial succinate dehydrogenase (SDH) activity in rat oral carcinogenesis and its biological significance. Methods: Rats with tongue cancer were induced by 2 × 10-5 4-nitroquinoline-1-oxide (4NQO) drinking water for 9 to 32 weeks in Wistar rats. Tongue specimens from 30 different pathological stages Dorsal tissue mitochondria, microplate reader SDSD enzyme activity changes. Results: SDH activity of mitochondria decreased gradually in rat tongue leukoplakia. SDH activity in squamous cell carcinoma was significantly lower than that in normal mucosa (P <0.05). SDH gene expression and enzyme activity trends are more consistent. CONCLUSION: The decrease of SDH activity and m RNA expression is associated with the occurrence and development of oral cancer. The TCA cycle may play an important role in the carcinogenesis of oral mucosa.
其他文献
Interdecadal and interannual variations of saline-alkali land area in Qianan County, Jilin Province, China were comprehensively analyzed in this paper by means
This study sought to contribute to an improved understanding of soil erosion and redistribution on Mediterranean agricultural land,where traditional soil conser
In order to improve the yield and stability of ferrate in solution, dissolved Fe (VI) prepared with NaOH and KOH respectively was compared in this study. The re
A genetic-fuzzy HEV control strategy based on driving cycle recognition (DCR) was built. Six driving cycles were selected to represent different traffic conditi
采用实时荧光定量PCR和高效液相色谱技术对太湖南泉水域2009年5月至12月产毒微囊藻的环境丰度及其微囊藻毒素-LR产毒能力进行研究,并监测水质和富营养化程度.研究结果表明:南
越来越多的证据提示肿瘤细胞内紊乱的泛素化过程调控了肿瘤的发生发展。泛素结合酶E2C（ ubiquitin-conjugating enzymeE2C， UBE2C）是泛素结合酶家族的重要成员，是细胞有丝分裂中
Three new species of Chresmodidae are described. They belong to two different genera:Chresmoda multinervis sp. nov., Chresmoda shihi sp. nov. and Jurachresmoda
As the most important ecosystem in Beijing, the forest supports a lot of ecosystem services to local and around communities, which plays a key role in the maint
目的:观察尼古丁对大鼠牙槽骨内碱性磷酸酶(ALP)和牙本质基质蛋白1(DMP1)C末端表达的影响。方法:将20只5周龄健康雄性Wistar大鼠随机等分为实验组和对照组,每组再随机等分为3
Spatial and temporal distributions of nutrients (N and P) and heavy metals (Cu, Zn, Pb and Cd) in the intertidal sediments at Dongtan wetland of the Changjiang