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分别采用连续4周喂养5%乙醇、连续14d水平台环境饲养法以及暗箱饲养模拟全夜状态等方法建立慢性睡眠剥夺模型,观察三种方法诱导睡眠障碍对小鼠免疫功能的抑制作用。通过脾脏称重、FACS法检测外周血CD3+、CD4+和CD8+T细胞数变化、ELISA法检测血清中IL-2、TNF-α以及B细胞产生抗牛血清白蛋白(BSA)特异性抗体含量、LDH法测定NK细胞杀伤功能等方法检测实验小鼠免疫功能变化。实验结果显示:与正常对照组相比,实验组免疫指标均有不同程度改变,包括CD3+、CD4+和CD8+T细胞数减少;IL-2分泌量下降和TNF-α分泌量升高;NK杀伤功能下降;脾重减轻;抗BSA特异性抗体分泌量下降。实验证明,长期5%乙醇摄入、水平台以及暗环境干扰引起的慢性睡眠障碍对机体的免疫功能具有明显抑制作用。
Chronic sleep deprivation models were established by feeding 5% ethanol continuously for 4 weeks, simulating the feeding environment for 14 days continuously, and simulating the overnight state of black box feeding. The inhibitory effects of three methods on inducing sleep disturbance on immune function were observed. The number of CD3 +, CD4 + and CD8 + T cells in peripheral blood was measured by FACS and the levels of anti-bovine serum albumin (BSA) -binding antibodies were measured by ELISA. LDH assay of NK cell killing function and other methods to detect changes in immune function in experimental mice. The experimental results showed that compared with the normal control group, the immunological indexes of the experimental group changed in varying degrees, including the decrease of the number of CD3 +, CD4 + and CD8 + T cells; the decrease of the secretion of IL-2 and the increase of the secretion of TNF- Decreased function, reduced spleen weight and decreased secretion of anti-BSA-specific antibodies. Experiments show that long-term 5% ethanol intake, water table and dark environment caused by chronic sleep disturbances have a significant inhibitory effect on the immune function.