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目的：为了解真皮鞘成纤维细胞的生物学特征。方法：采用酶消化法、组织块法分别进行毛囊真皮鞘成纤维细胞培养。结果：２种方法均获成功。培养的真皮鞘成纤维细胞具有聚集生长的特征，胞体较大，一般有２ 个突起；酶消化培养法操作简便、省时，但容易混有其它成纤维细胞；组织块法培养的细胞虽然培养速度较慢，但纯度较高。结论：组织块法是进行毛囊真皮鞘成纤维细胞研究较理想的培养方法。
Objective: To understand the biological characteristics of dermal sheath fibroblasts. Methods: Hair follicle dermal sheath fibroblasts were cultured by enzyme digestion and tissue block method respectively. Results: Both methods were successful. Cultured dermal sheath fibroblasts have the characteristics of aggregative growth, large cell body, usually 2 protrusions; enzyme digestion and culture method is simple, time-saving, but easily mixed with other fibroblasts; tissue culture of cells cultured cells Slower, but higher purity. Conclusion: The tissue block method is an ideal culture method for hair follicle dermal sheath fibroblasts.