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目的建立关苍术高效液相色谱指纹图谱并与茅苍术、北苍术和朝鲜苍术的指纹图谱进行比较。方法色谱柱为Promosil C_(18)(250 mm×4.6 mm,5μm)柱,流动相为乙腈-体积分数5‰磷酸水,梯度洗脱,检测波长为220 nm,流速为1.0 m L·min~(-1)。结果建立了关苍术指纹图谱,共标定22个共有峰,通过与对照品色谱图进行比对,对其中的8个色谱峰进行了指认。同时也建立了另三种苍术指纹图谱。四种苍指纹图谱差异较明显。结论建立了关苍术的高效液相指纹图谱,有助于深入研究、评价关苍术药材的质量。
OBJECTIVE To establish HPLC fingerprint of Kansu herb and compare its fingerprints with that of Atractylodes japonica, Atractylodes japonica and Korean herb. Methods The column was a Promosil C_ (18) (250 mm × 4.6 mm, 5 μm) column with a mobile phase of acetonitrile - 5% phosphoric acid with a gradient elution at a detection wavelength of 220 nm and a flow rate of 1.0 m L · min ~ (-1). Results A total of 22 common peaks of Guanzhu were established and compared with the reference chromatograms. Eight of them were identified. At the same time also established three other herb fingerprints. Four kinds of pale fingerprints more obvious differences. Conclusion The establishment of high-performance liquid chromatography fingerprints of herbs, help to further study, evaluate the quality of Guan herb medicine.