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目的对非霍奇金淋巴瘤的微小病灶（ＭＲＤ）及时发现。方法应用聚合酶链反应－聚丙烯酰胺凝胶电泳（ＰＣＲ－ＰＡＧＥ）和银染色法对２１１例非霍奇金淋巴瘤（ＮＨＬ）进行免疫球蛋白重链基因（ＩｇＨ），Ｔ细胞受体γ链基因（ＴＣＲγ）的克隆性重排检测。结果重排之ＩｇＨ为１０８例（５１．２％），ＴＣＲγ４５例（２１．３％），双阳性１２例（５．７％），阴性４６例（２１．８％）。病理型别恶性程度愈高，ＴＣＲγ及双阳性愈高。原发于鼻及咽淋巴环的ＮＨＬ比胃肠及周围淋巴结的ＴＣＲγ及双阳性高。ＴＣＲγ及双阳性的增高与病情恶化程度呈正相关。结论；以这种检测方法来指导化疗可以提高生存率和治愈率
Objective To detect the minute lesion (MRD) of non-Hodgkin’s lymphoma in time. Methods Immunoglobulin heavy chain gene (IgH) and T cell receptor γ were performed on 211 cases of non-Hodgkin lymphoma (NHL) by polymerase chain reaction-polyacrylamide gel electrophoresis (PCR-PAGE) and silver staining. Clonal rearrangement of the TCRγ gene. Results The rearrangement of IgH was 108 cases (51.2%), TCRgamma was 45 cases (21.3%), double positive was 12 cases (5.7%), and negative was 46 cases (21.8%). The higher the degree of pathological malignancy, the higher the TCRγ and double positives. The NHL originating in the nasal and pharyngeal lymphatic rings was higher than the TCRγ and double positive in the gastrointestinal and peripheral lymph nodes. The increase of TCRγ and double positive was positively correlated with the severity of the disease. Conclusion: The use of this assay to guide chemotherapy can improve survival and cure rates