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天然丝素蛋白由于其良好的生物相容性而成为一种具有良好应用前景的生物材料,但其实际应用受制于其再生生的水溶性及较差的力学性能。添加其他高分子材料提高材料力学性能会影响其生物相容性,通过甲醇处理可以改善其不溶性,但甲醇的毒性可能会影响其生物应用。报道一种直接制备不溶性纯丝素薄膜而不需要甲醇处理的方法。傅里叶红外(FTIR)和X射线衍射(XRD)光谱表明随浓度的增加,薄膜中的β折叠构象增加,当浓度为15%,干燥温度为60℃时,材料中的长程α螺旋构象基本消失,薄膜基本不溶于水。此条件下制备的丝素蛋白薄膜具有优异的湿态机械性能,最高拉伸强度和断裂伸长率可以达到15.9 Mpa和49.4%。
Due to its good biocompatibility, natural silk fibroin has become a promising biomaterial, but its practical application is limited by the water-soluble and poor mechanical properties of its regeneration. Adding other polymer materials to improve the mechanical properties of the material can affect its biocompatibility and its insolubility can be improved by methanol treatment, but its toxicity may affect its biological application. A method of directly preparing an insoluble pure silk fibroin membrane without the need for methanol treatment is reported. Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) spectroscopy showed that with increasing concentration, the β-sheet conformation increased in the film. When the concentration was 15% and the drying temperature was 60 ℃, the long- Disappear, the film is basically insoluble in water. The silk fibroin films prepared under these conditions have excellent wet mechanical properties with the highest tensile strength and elongation at break of up to 15.9 Mpa and 49.4%.