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目的建立一种早期、快速诊断庚型肝炎的血清学检测方法。方法以辣根过氧化物酶标记庚型肝炎病毒（ＨＧＶ）多肽ＮＳ３，ＮＳ５区段抗原，建立了捕获酶联免疫吸附试验（ＥＬＩＳＡ），用于检测血清中ＨＧＶＩｇＭ抗体。结果本法不受特异性ＩｇＧ的竞争和类风湿因子的干扰；与其它致肝炎的病毒（ＨＡＶ、ＨＢＶ、ＨＣＶ、ＨＥＶ、ＣＭＶ、ＥＢＶ）无交叉反应。检测４６例非甲、乙、丙、戊型肝炎患者血清，抗－ＨＧＶＩｇＭ阳性１４例，阳性率３０．４３％，其中，６例同时为ＨＧＶＲＮＡ阳性，阳性符合率为４２．８６％（６／１４），检测１２例庚型肝炎病人双份血清，其中，急性期血ＨＧＶＩｇＭ抗体均为阳性。结论该法检测ＨＧＶＩｇＭ抗体特异性强，敏感性高，且简便快速，适用于临床对庚型肝炎新近感染的早期诊断，有推广应用价值。
Objective To establish an early and rapid serological test for the diagnosis of hepatic G-type hepatitis. Methods Capture enzyme-linked immunosorbent assay (ELISA) was used to label HGV polypeptide NS3 and NS5 antigen with horseradish peroxidase (HRP) for the detection of HGVIgM antibody in serum. Results The method was not affected by specific IgG competition and rheumatoid factor; no cross-reactivity with other hepatitis-causing viruses (HAV, HBV, HCV, HEV, CMV, EBV). Among the 46 patients with non-A, B, C and E hepatitis, 14 were positive for anti-HGVIgM, the positive rate was 30.43%. Among them, 6 were positive for HGVRNA and the positive coincidence rate was 42.86% 14). Twelve serum samples of patients with G hepatitis were detected. Among them, HGVIgM antibodies were all positive in acute phase. Conclusion The method of detecting HGVIgM antibody is highly specific, sensitive and simple and rapid. It is suitable for the early diagnosis of recent hepatitis G infection and has the value of popularization and application.