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目的 :为摸清我省莱姆病生物媒介—蜱的种群、分布、消长及其带菌情况 ,研究防制方法。方法 :用布旗法及动物体外捕捉法收集蜱 ,分类鉴定 ,并进行季节消长调查 ;应用直接荧光抗体染色法查蜱中肠带菌率 ,BSK培养基分离病原体。现场喷洒毒死蜱和高效氯氰菊酯 ,比较处理前后及处理组与对照组蜱密度变化情况 ;使用避蚊胺作实验室和现场驱蜱效果观察。结果 :我省长江南北均有蜱存在 ,隶属 1科 3属 5种 ;蜱密度有明显的季节性。二棘血蜱中肠带螺旋体率为13.0 % ;捕捉活蜱 4 0 0 0多只作螺旋体分离 ,未获阳性培养物。现场喷洒 0 .5%毒死蜱和 0 .0 4 5%高效氯氰菊酯 ,可将蜱杀灭 ,持效在 30d以上 ;用同样的药物对动物及其圈舍喷洒 ,2 4h可杀灭所有的蜱 ;避蚊胺实验室驱蜱效果在 4h以上 ,现场效果达 3h。结论 :蜱在我省广泛存在 ,现场喷洒毒死蜱、高效氯氰菊酯及使用避蚊胺 ,对蜱有较好的防制和驱避效果
OBJECTIVE: To find out the population, distribution, growth and decline of the tick-borne tick-borne Lyme disease carrier in our province and to study the control methods. Methods: Ticks were collected by cloth flag method and animal in vitro capture method. The ticks were classified and identified, and the seasonal fluctuation was investigated. The rate of midgut ticks was detected by direct fluorescent antibody staining, and the pathogens were isolated by BSK medium. Spraying chlorpyrifos and beta-cypermethrin on the spot, comparing the changes of tick density before and after treatment and the treatment group and the control group; Results: There were all ticks in north and south of Yangtze River in our province, belonging to 3 genera and 3 genera and 5 species. The tick density was obviously seasonal. The rate of spirochaete in snakehead ticks was 13.0%. More than 4000 live ticks were only isolated from spirochetes, and no positive culture was obtained. Spraying 0.5% chlorpyrifos and 0.045% alpha-cypermethrin on-site can kill the ticks for more than 30 days. Spraying the animals and their pens with the same drugs can kill all the ticks within 24 hours. Mosquito repellent laboratory ticks tick effect in more than 4h, the effect of up to 3h. CONCLUSION: Ticks are widespread in our province. Spraying chlorpyrifos, beta-cypermethrin and DEET on the spot have better control and repellent effects on ticks