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目的：在藻兰蛋白原有分离纯化方法基础上，探讨对其进行改进的可能性。方法：用50％（W／V）硫酸倭沉淀法粗分离的藻兰蛋白，再用DEAE离子交换柱和羟基磷灰石柱纯化。结果：藻兰蛋白纯度达到92％，并在619.4mm处有最大吸收峰。在聚丙烯改胺凝胶电泳（PAGE）上只显示一条带。分子量约为26KD。结论：该方法既可达到较高纯度，又适合大量生产。
OBJECTIVE: To explore the possibility of improvement of phycocyanin protein based on its original method of isolation and purification. Methods: The crude protein was separated by 50% (W/V) barium sulfate precipitation and then purified by DEAE ion exchange column and hydroxyapatite column. Results: The purity of phycocyanin reached 92%, and there was a maximum absorption peak at 619.4 mm. Only one band was shown on polyacrylamide gel electrophoresis (PAGE). The molecular weight is about 26KD. Conclusion: This method can achieve both high purity and mass production.