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用流式细胞仪对51例大肠癌患者的癌中心灶、癌旁组织和淋巴结转移灶263份样品细胞进行DNA含量测定。结果表明,各组均出现了DNA异倍体病例,癌中心灶、癌旁组织和淋巴结检出率分别为72.6%、25.2%和22.2%。在14例二倍体肿瘤中有5例癌旁组织中出现了异倍体细胞。淋巴结转移灶DI值与癌中心灶有良好的一致性,而癌旁组织与癌中心灶的一致性较差。在癌中心灶不同倍体类型中,异倍体肿瘤的浸润及转移能力明显高于其它倍体肿瘤。
Flow cytometry was used to determine the DNA content of 263 samples of 51 patients with colorectal cancer, including cancer foci, paracancerous tissues, and lymph node metastases. The results showed that there were cases of DNA aneuploids in each group, and the detection rates of cancer foci, adjacent tissues, and lymph nodes were 72.6%, 25.2%, and 22.2%, respectively. Aneuploid cells appeared in 5 paracancerous tissues in 14 cases of diploid tumors. The DI value of lymph node metastases was in good agreement with the focus of the cancer, and the consistency between the adjacent tissues and the cancer focus was poor. In the different ploidy types of cancer foci, aneuploid tumors were significantly more invasive and metastatic than other ploidy tumors.