目的:建立高效液相色谱分析方法测定血浆中依米配能浓度。方法:血样经甲醇沉淀蛋白,以甲醇硼酸(0.2mol·L-1,pH7.14)(3∶97)为流动相,紫外313nm处检测。结果:在1.8～60μg·ml-1浓度范围内,峰面积与浓度呈良好线性关系,r=0.9992,最低检测浓度为1.8μg·ml-1,平均回收率(98.0±5.4)%。结论:该方法快速、简便、灵敏、可行。 Objective: To establish a HPLC method for the determination of plasma concentration of Eplerenone. Methods: The blood samples were precipitated with methanol, and the mobile phase was methanol boric acid (0.2mol·L-1, pH7.14) (3:97). Results: In the concentration range of 1.8 ~ 60μg · ml-1, there was a good linear relationship between peak area and concentration, r = 0.9992, the lowest detection concentration was 1.8μg · ml-1, the average recovery was 98.0 ± 5.4%. Conclusion: This method is rapid, simple, sensitive and feasible.