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为了了解食管癌的发生发展与机体免疫性的关系,摘除新生 A 系小鼠的胸腺,以降低机体的细胞免疫反应能力,籍以观察其对甲基苄基亚硝胺(MBNA)(1毫克/公斤体重/日)诱发小鼠前胃(被认为是食管的延伸部分)上皮癌变的影响,以及淋巴网状组织的改变。摘除胸腺的手术系参考 Miller 及 Parrott 二氏的方法,加以简化,并利用解剖显微镜以保证胸腺摘去的完全。出于手术的成功率(指术后经组织学检查胸腺确实去尽的)较低,对200多只新生鼠的进行了去胸腺手术,结果,只有9只合乎要求。胸腺去尽的小鼠,有4/9例出现消耗性疾病(Wastiny disease),另有5/9例则否。不管出现与否,其共同特点是全身淋巴网状组织中的小淋巴细胞显著丢失。但出现消耗性疾病的小鼠,消耗性体征的表现与淋巴组织的丢失要明显得多。胸腺去尽并出现消耗性疾病的小鼠,MBNA 诱发的前胃乳头状瘤和早期癌的潜伏期,似较单纯给 MBNA 的对照组要短。但两组的肿瘤发生率无明显差异。在只灌 MBNA 的对照组小鼠淋巴结内,有8/13例出现了“窦组织细胞增生”,其中,5/13例前胃肿瘤病变的“炎灶”(指上皮与固有膜中淋巴细胞和单核细胞的灶性浸润)明显;而胸腺去尽的小鼠仅有1/9例淋巴结内出现 SH;前胃上皮“炎灶”多数也不明显,说明“SH”与“炎灶”的出现可能与机体的抵抗力有关。在胸腺去尽的小鼠中,有一部分发现有垂体前叶嗜酸性细胞颗粒减少和肾上腺皮质网状带消失的现象。
In order to understand the occurrence and development of esophageal cancer and the relationship between the body immunity, the newborn A Department of mouse thymus removed to reduce the body’s cellular immune response, Ji membership to observe its methyl benzyl nitrosamine (MBNA) (1 mg / Kg body weight / day) induces epithelial canceration in the anterior stomach of the mouse (an extension of the esophagus) and changes in lymphoreticular tissue. Thymectomy surgery Department reference Miller and Parrott two methods, to be simplified, and the use of dissecting microscope to ensure the removal of thymus complete. Out of more than 200 newborn rats undergoing surgery for thymus, the success rate of surgery (which means that the thymic gland was actually eliminated after the surgery) was low, and as a result, only 9 were satisfactory. Thymic-depleted mice, 4/9 cases of wasting disease (Wastiny disease), and another 5/9 cases no. Regardless of whether they occur or not, their common denominator is the significant loss of small lymphocytes in the generalized lymph reticular tissue. However, mice with wasting disease showed significantly more consumptive signs and lymphoid tissue loss. Mice with depleted thymus and wasting disease appeared to have shorter incubation periods for MBNA-induced papilloma and early cancers than did MBNA alone. However, there was no significant difference in the incidence of tumors between the two groups. There were 8/13 “sinusoidal hyperplasia” in the lymph nodes of control mice injected with MBNA alone. Among them, 5/13 of the “focal lesions” of the anterior gastric tumor (referred to as epithelial and intrinsic membrane lymphocytes And focal infiltration of monocytes) was evident; while only 1/9 of thymus mice developed SH in the lymph nodes; anterior gastric epithelium, “inflammation” most are not obvious, indicating that “SH” and “inflammation” The emergence may be related to the body’s resistance. In thymus mice, a part of the anterior pituitary cells found eosinophilic granulocytes and adrenal reticular disappearance of the phenomenon.