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目的建立HPLC同时测定麻黄及其制剂中3种生物碱含量。方法以乙腈-甲醇-0.1%磷酸溶液(1.5∶4∶94.5)为流动相,同时测定麻黄、金麻杏止咳片和麻杏止咳片中3种生物碱的含量。结果盐酸麻黄碱、盐酸伪麻黄碱、盐酸甲基麻黄碱的进样量分别为0.102 9~0.686,0.025 11~0.167 4,0.022 23~0.148 2μg,分别与峰面积呈良好的线性关系;平均回收率分别为100.56%,97.84%和98.42%,RSD分别为0.20%、0.94%和1.31%(n=6)。结论所建立的HPLC可通过测定3种生物碱的含量综合评价麻黄及其制剂的质量。
Objective To establish HPLC simultaneous determination of Ephedra and preparations of three kinds of alkaloid content. Methods The contents of three alkaloids in Ephedra, Jinmao Xingxue Tablet and Maxing Cough Tablets were determined by acetonitrile-methanol-0.1% phosphoric acid solution (1.5: 4: 94.5) Results The injection volumes of ephedrine hydrochloride, pseudoephedrine hydrochloride and methyl ephedrine hydrochloride were 0.102 9 ~ 0.686,0.025 11 ~ 0.167 4,0.022 23 ~ 0.148 2μg, respectively, with a good linear relationship with the peak area respectively. The average recoveries were 100.56%, 97.84% and 98.42% respectively, with RSDs of 0.20%, 0.94% and 1.31%, respectively (n = 6). Conclusion The established HPLC can be used to evaluate the quality of ephedra and its preparations by measuring the content of three alkaloids.