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目的研究P53、nm23-H1和MMP-9基因在大肠癌中的表达及其与患者的临床病理和预后的关系。方法采用免疫组化LSAB法检测78例大肠癌标本及30例正常粘膜的P53、nm23-H1和MMP-9的基因蛋白表达。结果(1)P53、nm23-H1、MMP-9的阳性表达率在大肠癌中分别为56.41%、39.74%和58.97%,在正常肠粘膜中的表达率分别为13.33%、83.33%和6.67%,其差别有显著性意义(P<0.01)。(2)P53和MMP-9的表达与肿瘤的浸润深度和淋巴结转移呈正相关(P<0.01),nm23-H1的表达与肿瘤的浸润深度和淋巴结转移呈负相关(P<0.01)。结论联合检测大肠癌中P53、nm23-H1和MMP-9基因表达,对了解大肠癌的生物学特性和预后判断有重要意义。
Objective To investigate the expression of P53, nm23-H1 and MMP-9 genes in colorectal cancer and their relationship with clinicopathologic features and prognosis. Methods The expression of P53, nm23-H1 and MMP-9 in 78 cases of colorectal cancer and 30 cases of normal mucosa were detected by immunohistochemistry. Results (1) The positive rates of P53, nm23-H1 and MMP-9 in colorectal cancer were 56.41%, 39.74% and 58.97%, respectively, and the expression rates in normal intestinal mucosa were 13.33%, 83.33% and 6.67%, respectively. The difference was significant (P<0.01). (2) The expression of P53 and MMP-9 was positively correlated with the infiltration depth and lymph node metastasis (P<0.01). The expression of nm23-H1 was negatively correlated with the infiltration depth and lymph node metastasis (P<0.01). Conclusion The combined detection of P53, nm23-H1 and MMP-9 gene expression in colorectal cancer is important for understanding the biological characteristics and prognosis of colorectal cancer.