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本文应用多聚酶链反应—单链构象多态性(PCR-SSCP)分析,对人宫颈癌(cervical carcinoma)和慢性宫颈炎(chronic cervicitis)组织内的P_(53)基因5-6、7-8、9外显子(exon)变异进行研究。结果表明:35例宫颈癌组织中8例出现了异常电泳带,总变异率22.86％(8/35)。其中,5-6外显子2例,变异率为5.71％(2/35);7-8外显子5例,变异率为14.29％(5/35),是变异率最高的基因片段;9外显子1例,变异率为2.86％(1/35)。8例变异标本中3例P_(53)5-6、7-8外显子同时发生突变,占变异标本的37.5％(3/8),占宫颈癌组织总标本的8.57％(3/35)。17例慢性宫颈炎组织P_(53)5-6、7-8、9外显子均未出现异常。提示:PCR-SSCP技术能有效地检出宫颈组织P_(53)基因5-9外显子变异的情况;P_(53)5-9变异是人宫颈癌发生发展过程中的一个重要事件和生物学行为;宫颈癌组织P_(53)变异多发生在5-8外显子,有不同外显子同时变异的现象;慢性宫颈炎组织无P_(53)5-6、7-8、9外显子变异现象,说明该病的发生发展与抑癌基因P_(53)外显子变异无关。
In this study, polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis of human cervical cancer (cervical carcinoma) and chronic cervicitis tissue P53 gene 5-6,7-8 , 9 exon variation were studied. The results showed that in 35 cases of cervical cancer, 8 cases appeared abnormal electrophoresis bands, the total mutation rate was 22.86% (8/35). The mutation rate was 5.71% (2/35) in 2 cases with 5-6 exons, 5 cases with 7-8 exon, the mutation rate was 14.29% (5/35) 9 exons in 1 case, the mutation rate was 2.86% (1/35). The mutation of P 53 53 5-6 and 7-8 exons in 8 of 8 samples were found to be mutation at the same time, accounting for 37.5% (3/8) of the total samples and accounting for 8.57% (3/35) of total samples of cervical cancer ). 17 cases of chronic cervicitis tissue P_ (53) 5-6,7-8,9 exons were not abnormal. Tip: The PCR-SSCP technique can effectively detect the mutation of 5-9 exon of P53 gene in cervical tissue. P53-5-9 mutation is an important event in the development of human cervical cancer and the biological (P53) in cervical cancer tissue occurred in exon 5-8 with different exons at the same time. Chronic cervicitis had no P53 (5-36) Exon mutation phenomenon, indicating that the occurrence and development of the disease and tumor suppressor gene P 53 exon mutation has nothing to do.