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目的:观察染料木黄酮(GEN)对人白血病细胞增殖及凋亡的影响,探讨其抗白血病的可能性。方法:不同浓度(10、20、30、40、50 mg/L),不同时间(6、12、24、48、72 h)染料木黄酮作用后,甲基噻唑基四唑(MTT)法检测K562细胞(慢性髓细胞性白血病细胞株)及人外周血单个核细胞增殖;DNA断裂梯带电泳、Annexin-V/PI双染色流式细胞仪分析检测GEN诱导K562细胞凋亡的作用。结果:GEN可有效地抑制肿瘤细胞增殖,而对正常细胞毒性较小(PBM-CIC50=35.5 mg/L和K562 IC50=13.2 mg/L),抑制作用呈明显的时间、剂量—效应关系(P<0.05);GEN体外能够诱导K562细胞凋亡,诱导作用呈时间、剂量依赖关系(P<0.05);结论:GEN具有抗白血病细胞的作用。
Objective: To observe the effect of genistein (GEN) on the proliferation and apoptosis of human leukemia cells and to explore the possibility of anti-leukemia. METHODS: Methyl thiazolyl tetrazolium (MTT) assay was used to detect the effects of genistein after different concentrations (10, 20, 30, 40 and 50 mg / L) K562 cells (chronic myeloid leukemia cell line) and human peripheral blood mononuclear cell proliferation; DNA fragmentation ladder electrophoresis, Annexin-V / PI double staining flow cytometry analysis of GEN-induced apoptosis in K562 cells. Results: GEN inhibited the proliferation of tumor cells effectively and had less cytotoxicity to normal cells (PBM-CIC50 = 35.5 mg / L and K562 IC50 = 13.2 mg / L). The inhibitory effect was significantly related to the dose- <0.05). GEN could induce apoptosis of K562 cells in vitro in a dose-dependent and time-dependent manner (P <0.05). Conclusion: GEN has the anti-leukemic effect.