论文部分内容阅读
Objective:To investigate the actions of cytokine profile in the immune cells by a seven amino acid peptide mimic from HVR1 of HCV (GQTYTSG,named 7P).Methods:The peripheral blood mononuclear cells (PBMCs) from 17 healthy blood donors were stimulated with 7P,and the cytokine levels in CD4+CD8-,CD4-CD8+ T cells,NK,NKT cells were analyzed by the intracellular cytokine staining.Results:The frequency of cells which secreted interferon-γ (IFN-γ) was found to be significantly increased in all cells,interleukin-10(IL-10) was significantly increased in CD4+CD8-,CD4-CD8+ T cells but decreased in NK,NKT cells,and tumor necrosis factor-α (TNF-α) was decreased in CD4+CD8-,CD4-CD8+ but increased in NK cells.Conclusion:7P could induce a cytokine profile in different immune cells in vitro and there was some difference between the CD4+CD8-,CD4-CD8+ T cells which represented the adaptive immunity cells and NK,NKT cells which represented the innate immunity cells.This kind of variation of cytokine profile might contribute to anti-virus and anti-inflammatory immune reaction.
Objective: To investigate the actions of cytokine profile in the immune cells by a seven amino acid peptide mimic from HVR1 of HCV (GQTYTSG, named 7P). Methods: The peripheral blood mononuclear cells (PBMCs) from 17 healthy blood donors were stimulated with 7P , and the cytokine levels in CD4 + CD8-, CD4-CD8 + T cells, NK, NKT cells were analyzed by the intracellular cytokine staining. Results: The frequency of cells which secreted interferon- γ (IFN- γ) was found to be significantly increased increased in all cells, interleukin-10 (IL-10) was significantly increased in CD4 + CD8-, CD4-CD8 + T cells but decreased in NK, NKT cells, and tumor necrosis factor- CD8-, CD4-CD8 + but increased in NK cells. Confc: 7P could induce a cytokine profile in different immune cells in vitro and there was some difference between the CD4 + CD8-, CD4-CD8 + T cells which represented the adaptive immunity cells and NK, NKT cells which represented the innate immunity cells. This kind of variation of cytokine pro file might contribute to anti-virus and anti-inflammatory immune reaction.