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采用主动回避法进行大鼠学习记忆训练 ,选出学习成绩好和差的大鼠 ,用原位杂交、免疫细胞化学结合图像分析方法观察nov基因表达的差异。结果显示 ,novmRNA和NOV蛋白阳性神经元主要分布于海马、扣带皮质和联合皮质锥体层、基底神经节和下丘脑等脑区。好成绩组NOV蛋白免疫反应最强 ,阳性细胞最多 ,差成绩组nov基因的表达比假性条件反射组的表达稍强。novmRNA的表达在各组之间无明显的差异。以上结果提示 ,nov基因可能参与学习记忆的调控过程 ,这种调控发生在NOV蛋白翻译水平。
The active avoidance method was used to train learning and memory of rats, and the rats with good and poor grades were selected. The differences of nov gene expression were observed by in situ hybridization and immunocytochemistry combined with image analysis. The results showed that nov mRNA and NOV positive neurons were mainly distributed in the hippocampus, cingulate cortex and cortex pyramidal layer, basal ganglia and hypothalamus and other brain regions. The NOV protein of the best grade group had the strongest immune response and the highest number of positive cells, while the nov gene expression of the poor grade group was slightly stronger than that of the pseudo-conditioned group. There was no significant difference in the expression of nov mRNA between the groups. The above results suggest that nov gene may be involved in the regulation of learning and memory, this regulation occurs in NOV protein translation level.