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本文通过体外培养人盲肠未分化腺癌(Hce-8693)细胞时,细胞数与培养上清液β_2-微球蛋白(β_2—m)浓度的关系;荷人直肠粘液腺癌裸鼠移植瘤模型肿瘤重量与血清中β_2-m浓度的关系;以及肠癌患者手术前后血清β_2-m浓度的变化来评估β_2-m浓度与肿瘤生长的相关性。 材料和方法 一、细胞培养:采用本院肿瘤研究所建立的人盲肠未分化腺癌(Hce—8693)细胞系作为实验对象。取对数生长期的细胞,按每ml1×10~5细胞数,共30ml30万细胞数接种在30ml方瓶中,共21瓶,按常规培养24h进行活细胞计数,计数后的培养瓶进行离心,取上清液0.1ml,用放射免疫分析法测定β_2-m浓度。以后每隔24h重复以上操作,连续7天,每天取3瓶,取平均值。对细胞数及相应的β_2-m浓度作直线回归,求出相关系数。
In this article, the relationship between the number of cells and the concentration of culture supernatant β2-microglobulin (β_2-m) in cultured human cecal undifferentiated adenocarcinoma (Hce-8693) cells, and the model of xenograft of human rectal mucinous adenocarcinoma in nude mice The relationship between tumor weight and serum concentration of β 2 -m, and the change of serum β 2 -m concentration before and after surgery in patients with colon cancer were used to evaluate the correlation between β 2 -m concentration and tumor growth. Materials and Methods 1. Cell culture: The human cecal undifferentiated adenocarcinoma (Hce-8693) cell line established by the Institute of Tumor Research in this hospital was used as the experimental object. Take cells in logarithmic growth phase and inoculate 30 ml cells per ml with 30 ml cells per ml in a 30 ml square flask for a total of 21 flasks. Viable cells were counted after 24 hours of routine culture, and the counted flasks were centrifuged. The supernatant was taken 0.1 ml, and the β 2 -m concentration was measured by radioimmunoassay. After repeating the above operations every 24 hours, take 3 bottles a day for 7 consecutive days and take the average. The cell number and the corresponding β 2 -m concentration were linearly regressed and the correlation coefficient was calculated.