目的探讨ERK1/2抑制剂PD98059体外抗细粒棘球绦虫原头蚴作用。方法体外培养细粒棘球绦虫原头蚴,分组后分别加入100、50、25、12.5μmol/L的PD98059后继续培养7d,利用伊红染色方法在倒置显微镜下观察原头蚴的活力,实验重复3次,绘制原头蚴活力曲线;扫描电子显微镜(SEM)下观察不同浓度PD98059组原头节表面结构改变。结果 PD98059作用1d对细粒棘球蚴原头节具有杀伤作用,第7d100μmol/L PD98059组原头节的活力仅为(12.4±0.9)%。超微结构显示原头节顶突外翻、变形,顶突界面缺损,吸盘变形,甚至出现虫蛀样损害。结论 PD98059在体外有显著的抗细粒棘球蚴原头节的作用,可认为是一种新型的抗包虫药物。 Objective To investigate the effect of ERK1 / 2 inhibitor PD98059 on protoscoiosis of Echinococcus granulosus in vitro. Methods Echinococcus granulosus was cultured in vitro. After the cells were treated with 100, 50, 25 and 12.5μmol / L PD98059 respectively, the cells were cultured for further 7 days. The protoscolex was observed under an inverted microscope with Eosin staining. Repeated 3 times to draw the prothalliolactone activity curve; scanning electron microscopy (SEM) under different concentrations of PD98059 group observed the surface structure of the original head section changes. Results PD98059 had a killing effect on the protoscoi of Echinococcus granulosus 1d on the 1st day. The viability of the protoscoleces of PD98059 group on the 7th day was only (12.4 ± 0.9)%. The ultrastructure showed that the protuberant protuberant eversion, deformation, apical interface defect, sucker deformation, and even wormhole-like damage. Conclusion PD98059 has significant anti-Echinococcus multilocularis activity in vitro and can be considered as a new anti-hydatid drug.