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将疫苗包裹在可生物降解的微球中 ,是一种极有潜力的新型疫苗载体系统 .用聚 -DL-乳酸 -聚乙二醇共聚物 ( PEL A)为材料 ,包裹乙型肝炎表面抗原 ( HBs Ag) ,制成缓释微球疫苗 ,以皮下注射或口服的方式免疫 BAL B/c小鼠 ,研究其免疫原性 .同时以乙肝常规铝佐剂疫苗免疫两剂作为对照 .结果表明 ,皮下注射单剂微球疫苗后 ,在第 1 4周 ,小鼠血清 Ig G滴度可达到与铝佐剂疫苗组相当的水平 ,维持较高的滴度 ;此外 ,口服微球疫苗组诱导的血清 Ig G滴度较低 ,但其 SIg A明显高于对照组和皮下注射微球疫苗组 ,可见 ,口服微球疫苗可诱导更高水平的粘膜抗体反应 .PELA微球作为疫苗载体系统是可行的 ,为研制新型疫苗提供了一条途径 .
The vaccine is encapsulated in biodegradable microspheres and is a promising new vaccine carrier system.With poly-DL-lactic acid-polyethylene glycol copolymer (PEL A) as the material, the hepatitis B surface antigen (HBs Ag) to make sustained-release microspheres vaccine, and BALB / c mice were immunized subcutaneously or orally to study its immunogenicity.At the same time, two doses of hepatitis A aluminum adjuvant vaccine were used as control.Results After subcutaneous injection of a single dose of microsphere vaccine, serum IgG titer in mice reached the level equivalent to the aluminum adjuvant vaccine group at the 14th week, maintaining a high titer. In addition, the oral microsphere vaccine group induced Of serum Ig G titers were lower, but SIg A was significantly higher than that of the control group and subcutaneous injection of microsphere vaccine group, it can be seen that the oral microsphere vaccine induced higher mucosal antibody response.PELA microspheres as a vaccine carrier system Feasible for the development of new vaccines provides a way.