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目的 :为获得编码高亲和力IgE受体(FcεR1)α链的cDNA序列。方法 :从正常人外周血中富集嗜碱性粒细胞 ,提总RNA进行RT PCR ,分子克隆 ,用自动测序及放射自显影测序。结果 :分离并鉴定了该cDNA重组子 ,在159密码子及185密码子分别发现了CAC→CGC与TAT→CAT置换。结论 :建立了一个编码FcεR1膜外区α链的cDNA重组子克隆 ,它不含引导肽序列 ,可能是一个来自中国人的该基因的变异体。
OBJECTIVE: To obtain the cDNA sequence coding for α-chain of high affinity IgE receptor (FcεR1). Methods: Basophils were enriched from normal human peripheral blood and total RNA was extracted for RT PCR and molecular cloning. Sequencing was performed by automated sequencing and autoradiography. RESULTS: The cDNA recombinant was isolated and identified. The CAC → CGC and TAT → CAT substitutions were found at codon 159 and codon 185, respectively. Conclusion: A recombinant cDNA clone encoding the α chain of FcεR1 extracellular domain was constructed. It contains no leader sequence and may be a variant of this gene from Chinese.