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目的:观察针刺肺俞穴对新型抗癌药物紫杉醇在肺鳞状细胞癌小鼠体内分布趋势的影响,为化疗药物靶向给药的研究提供新思路,进一步验证针药并举协同增效的机理。方法:选用肺鳞状细胞癌小鼠36只,采用随机的方式,分为4组,即模型组、针刺肺俞组、紫杉醇药物组、针药结合组(针刺肺俞+紫杉醇药物组),每组6只。针刺治疗针、药、针药结合组,施以电针疗法,每天1次,连续5 d。紫杉醇以腹腔注射给药,最后1次针刺后立即给药。给药后1、2、8、12、24 h每一时间点取6只小鼠肺、肝脏组织。用ELISA法进行各脏器P糖蛋白表达分析。结果:针刺、紫杉醇、针刺联合紫杉醇均能抑制肿瘤生长,具有良好的抑瘤作用。针剌联合紫杉醇抑瘤效果更加显著。结论:针刺联合紫杉醇治疗有更显著的抗肿瘤作用,并能改善实验动物的生存状态,针药结合对肺癌小鼠的治疗效果明显优于单一疗法。
Objective: To observe the influence of acupuncture at Feishu acupoint on the distribution trend of paclitaxel in lung squamous cell carcinoma in mice, provide new ideas for the targeted drug delivery of chemotherapy, and further verify the synergistic effect of acupuncture and medicine mechanism. Methods: Thirty-six lung squamous cell carcinoma (SCC) mice were randomly divided into four groups: model group, acupuncture group Feishu group, paclitaxel group, acupuncture group (acupuncture group Feishu group + paclitaxel group) ), 6 in each group. Acupuncture treatment of acupuncture, medicine, acupuncture and medicine combined group, administered electro-acupuncture, once a day for 5 days. Paclitaxel intraperitoneal injection, the last administration after acupuncture. At 1, 2, 8, 12 and 24 h after administration, 6 mice lung and liver tissues were taken at each time point. The expression of P glycoprotein in various organs was analyzed by ELISA. Results: Acupuncture, paclitaxel and acupuncture combined with paclitaxel can inhibit tumor growth and have good anti-tumor effect. Acupuncture combined paclitaxel inhibition effect is more significant. CONCLUSION: Acupuncture combined with paclitaxel has more significant anti-tumor effect and can improve the survival status of experimental animals. The therapeutic effect of acupuncture combined with paclitaxel on lung cancer mice is obviously better than monotherapy.