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[目的]通过检测人肝细胞癌组织中肿瘤相关巨噬细胞的浸润水平和特点,探讨肿瘤相关巨噬细胞在肝癌发生、进展中的作用。[方法]以CD68分子为巨噬细胞标志,采用免疫组织化学法检测116例肝细胞癌肿瘤组织巨噬细胞的浸润水平和特点。[结果]肝细胞癌组织中CD68阳性表达率为94.8%(110/116),而癌旁组织为17.2%(20/116)。巨噬细胞的浸润水平在不同TNM分期、是否存在血管侵袭、淋巴结转移及微卫星病灶分组间均有统计学差异(P<0.05),与患者预后呈负相关(P=0.001)。[结论]人肝细胞癌组织肿瘤相关巨噬细胞呈高浸润状态,且浸润水平与多项临床病理特征和预后密切相关,提示其可能参与了肝癌的发生、进展。
[Objective] To investigate the role of tumor-associated macrophages in the occurrence and progression of hepatocellular carcinoma (HCC) by detecting the level and characteristics of tumor-associated macrophages in human hepatocellular carcinoma. [Method] With CD68 as macrophage marker, the infiltration and characteristics of macrophage in 116 hepatocellular carcinoma tissues were detected by immunohistochemistry. [Results] The positive rate of CD68 in hepatocellular carcinoma was 94.8% (110/116), while that in paracancerous tissues was 17.2% (20/116). The infiltration of macrophages in different TNM stages, whether there is vascular invasion, lymph node metastasis and microsatellite loci between groups were statistically significant (P <0.05), and prognosis was negatively correlated (P = 0.001). [Conclusion] The tumor-associated macrophages of human hepatocellular carcinoma are highly infiltrated, and the infiltration level is closely related to many clinicopathological features and prognosis, suggesting that they may be involved in the occurrence and progression of hepatocellular carcinoma.