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目的建立不同来源的千金藤植物中千金藤素的HPLC测定方法。方法采用Angela C18色谱柱(250 mm×4.6 mm,5μm);流动相:乙腈–水(52∶48,含0.5%三乙胺、0.01%磷酸);检测波长:282 nm;柱温:30℃;体积流量:1.0 m L/min;进样体积:20μL。结果千金藤素质量浓度在9~216μg/m L时与峰面积值呈良好的线性关系(r=0.999 7),平均回收率为96.0%,RSD值为2.0%(n=6)。产自湖南的千金藤(No.13)、产自广西的白药子(No.17)中千金藤素的量较高,分别为0.013 9%、0.122 1%;同一植株的不同用药部位(No.12~15)中千金藤素的量也不尽相同,湖南产千金藤中千金藤素主要分布在茎中,其质量分数为0.013 9%。结论该方法操作简便、准确、重复性好,可用于千金藤植物千金藤、地不容和白药子中千金藤素的测定。
Objective To establish a HPLC method for the determination of Cepharanthine in Celastrus species from different sources. The method was carried out on an Angela C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-water (52:48, containing 0.5% triethylamine and 0.01% phosphoric acid) ; Volume flow: 1.0 m L / min; injection volume: 20 μL. Results The concentration of cepharanthine had a good linear relationship with the peak area (r = 0.999 7) when the concentration was 9-216 μg / mL, the average recovery was 96.0% and the RSD was 2.0% (n = 6). Ceratoides from Hunan (No. 13) and Baiqi Zi from Guangxi (No.17) had a high content of Cefradine 0.013 9% and 0.122 1%, respectively. No treatment sites of the same plant (No .12 ~ 15) in the amount of cepharanthine is also different, the Hunan thirteenth elm cedar root mainly distributed in the stem, the mass fraction of 0.013 9%. Conclusion The method is simple, accurate and reproducible. It can be used for the determination of Cepharanthine in Celastrus oryzae.