目的:观察C57小鼠ASCs(Adipose-derived stem cells,ASCs)体外培养的生物学特性,探讨其成脂成骨诱导分化能力。方法:无菌条件下切取C57小鼠腹股沟处脂肪组织,0.25%I型胶原酶消化,分离培养ASCs,37℃,5%CO2饱和湿度孵箱培养,细胞融合达80%时消化传代。观察其细胞形态;MTT比色法测细胞生长曲线;流式细胞仪检测细胞表面标志物;取第2代细胞行成骨及成脂诱导培养,3周后分别茜素红染色和油红O染色鉴定。结果:从C57小鼠脂肪组织中分离获取的ASCs呈长梭形,成纤维细胞样。细胞生长曲线呈“S”型,证明ASCs具有很强的增殖能力;流式细胞术分析结果:CD29、CD44、CD90阳性表达,CD31、CD34、CD45阴性表达;成骨诱导后茜素红染色阳性,成脂诱导后油红O染色阳性。结论:本试验分离培养的细胞为ASCs,具有确切分化能力。 OBJECTIVE: To observe the biological characteristics of cultured Adipose-derived stem cells (ASCs) in vitro and to explore their ability to differentiate into adipogenic osteoblasts. Methods: Adenocarcinoma of the C57 mice was excised under sterile conditions. The cells were digested with 0.25% collagenase I and isolated and cultured ASCs. The cells were incubated at 37 ° C with 5% CO2 in a humidified incubator. The cells were passaged at 80% confluency. The cell morphology was observed by MTT colorimetric assay. The cell surface markers were detected by flow cytometry. The second generation of cells were induced to osteoblast and adipogenic induction. After three weeks, alizarin red staining and oil red O Dye identification. Results: The ASCs isolated from adipose tissue of C57 mice were long fusiform and fibroblast-like. The results of flow cytometry showed that the positive expression of CD29, CD44 and CD90, negative expression of CD31, CD34 and CD45; the expression of alizarin red after osteogenic induction Positive staining, fat-induced oil red O staining. Conclusion: The cells cultured in this experiment are ASCs with definite differentiation ability.