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过继性免疫治疗是肿瘤生物治疗的重要手段之一.80年代后期,开始进行LAK细胞抗肿瘤过继免疫治疗,并取得一定效果.近来,γδT细胞和NK细胞在非恃异性或半特异性免疫治疗中的作用逐渐被重视.我们通过体外分离和增殖培养γδT细胞、NK细胞和LAK细胞,比较研究了3种细胞的抗肿瘤的生物学特性,结果显示,以MACS分离γδT细胞,培养2周后细胞数扩增600～800倍,CD3,CD8和γδT表达阳性率分别是70.41%,42.69%和40.5%,γδT细胞对NK敏感细胞K562以及NK不敏感细胞Raji,XG-73种不同到细胞均有较高的杀伤率,分别为35.98%,32.27%和49.08%,高于对照组PBMC的19.56%,11.34%和10.58%;NK细胞对此3种细胞的杀伤率分别是45.21%,12.34%和11.94%;
Adoptive adoptive immunotherapy is one of the important means of tumor biological treatment.At the late 1980s, anti-tumor adoptive immunotherapy of LAK cells began, and achieved some results.Recently, γδT cells and NK cells in the opposite sex or semi-specific immunotherapy We gradually cultured γδT cells, NK cells and LAK cells by in vitro isolation and proliferation, and compared the biological characteristics of the three kinds of cells in anti-tumor. The results showed that γδT cells were separated by MACS and cultured for 2 weeks The positive rates of CD3, CD8 and γδT were 70.41%, 42.69% and 40.5% respectively after 600-800 times of cell proliferation. The effects of γδT cells on NK sensitive cells K562 and NK-sensitive cells Raji and XG-73 were different from those of cells Higher killing rates were 35.98%, 32.27% and 49.08% respectively, which were 19.56%, 11.34% and 10.58% higher than PBMCs of control group. The killing rates of NK cells to these three kinds of cells were 45.21% and 12.34% And 11.94% respectively;