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目的:湿阻中焦证的发生有多种因素,其中,脾运化不足为主因.基于肺与脾在津液的输布与排泄上协调配合,在肺为水之上源的理论指导下,实验研究湿阻中焦证对肺水通道蛋白表达的影响,以揭示肺与脾在水液代谢及病理方面的相关性.方法:模拟外湿过盛、困阻脾胃,饮食不节、湿从内生,情志不遂、气机受阻、水湿不运三大致湿病因,建立湿阻中焦证模型.用酶联免疫法(ELISA)测定肺组织中AQP1、AQP4、AQP8的含量及中药干预后的变化.结果:造模前后水通道蛋白AQP1、AQP4表达有降低趋势,但无统计学意义,AQP8表达有显著降低;各给药组AQP1表达均呈升高趋势,不换金正气散高剂量及低剂量组AQP8表达均呈明显降低趋势.湿阻中焦证模型下,肺水通道蛋白的表达有一定改变,且中医中对湿阻中焦证具有治疗作用的方剂,对其改变有一定的调节作用.结论:水通道蛋白在肺中的表达,其在湿阻中焦证模型下的表达的改变,及中医方剂干预后其表达的改变,可能是脾主运化水饮、“脾气散精,上归于肺”、使水液正常输布功能的体现和分子基础之一,提示肺与脾在水液代谢方面存在一定的关联性.“,”Obhective:There are many factors of the source of syndrome of dampness in middle Jiao,the spleen deficiency is the main cause.The lung and spleen indistribution and excretion of body fluid is coordinated in the lung is based on lung as the source of waterunder the guidance of the theory,experimental study on the effect of the expression of syndrome of dampness in middleJiaoof Aquaporins in the lung,to reveal the lung and spleen in water metabolism and pathological correlation.Methods:Build a model of the syndrome of dampness in middle Jiao,simulating the three big etiological factors of retention of water and dampness:i.e.the functioning declination of spleen and stomach by exterior dampness luxus,interior dampness caused by intemperant diet,or stagnation of Qi caused by emotional depression.Measure the levels of AQP1,AQP4and AQP8in lung tissue and the changes of Chinese medicine after intervention by enzyme-linkedimmunosorbent assay (Elisa).Results:The expression of AQP1and AQP4was decreased in the model before and after modeling,but this difference is no significant.The expression of AQP8was significantly decreased in the model before and after modeling.The expression of AQP1 in all groups showed an upward trend.The expression of AQP8decreased obviously in the high dose and low dose groups of BuhuanjinzhengqiSan.The syndrome of dampness in middle Jiao,expression of Aquaporin in the lung is changed,and the prescription of Traditional Chinese Medicine forsyndrome of dampness in middle Jiaohas significant curative effect,have certain effect on the changes.Conclusion:There is a certain correlation between lung and spleen in water metabolism.Expression of Aquaporins in the lung,the change of the expression in syndrome of dampness in middle Jiao and the change of the expression in the intervention of Traditional Chinese Medicine,may be spleen governing transportation and transformation of water,“speen-qi on dispersing jing and the essence belongs to the lung” and one of the embodiment and the molecular basis of normal distribution function of liquid water.It suggests that the lung and spleen have some correlation in the metabolism of water.