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目的探讨黏液型铜绿假单胞菌药敏试验(K-B法)结果的报告时机,了解黏液型铜绿假单胞菌的分离及耐药性,为临床抗感染治疗提供依据。方法对所分离出的20株黏液型铜绿假单胞菌进行体外药敏试验(K-B法),记录不同时间段(24 h、48 h、72 h)的结果。再将此菌进行转种数代,使其黏液消失。将上述2次结果进行对比分析。结果黏液型铜绿假单胞菌24 h抑菌环直径与48 h、72 h差异有统计学意义(P<0.05)。黏液消失后的铜绿假单胞菌24 h、48 h、72 h的抑菌环直径差异无统计学意义(P>0.05),并与未转种前的黏液型铜绿假单胞菌48 h、72 h的结果差异无统计学意义(P>0.05)。结论黏液型铜绿假单胞菌的体外药敏试验(K-B法)结果应该尽量在48 h后报告,该菌对常用抗菌药物的体外药敏试验耐药性较低,但在临床用药时必须考虑黏液型铜绿假单胞菌在体内存在有生物膜的影响因素。
Objective To investigate the timing of reporting the results of the susceptibility test of mucopurulent Pseudomonas aeruginosa (K-B method), to understand the isolation and drug resistance of mucoid Pseudomonas aeruginosa, and to provide a basis for clinical anti-infective therapy. Methods The susceptibility tests of 20 strains of Mucous-type Pseudomonas aeruginosa were performed in vitro (K-B method). The results of different time periods (24 h, 48 h, 72 h) were recorded. Then the bacteria were transplanted for several generations to make mucus disappear. The above two results for comparative analysis. Results The diameter of bacteriostatic ring of mucoid Pseudomonas aeruginosa 24 h was significantly different from that of 48 h and 72 h (P <0.05). The diameter of bacteriostasis ring of Pseudomonas aeruginosa 24 hours, 48 hours and 72 hours after the mucus disappeared was not significantly different (P> 0.05), and with the non-transformant mucolytic Pseudomonas aeruginosa 48 hours, There was no significant difference in the results of 72 h (P> 0.05). Conclusion The results of in vitro susceptibility test (KB method) of mucoid Pseudomonas aeruginosa should be reported as soon as possible after 48 h. The antibacterial activity of commonly used antimicrobial agents in vitro is low, but it must be considered in the clinical application Mucous Pseudomonas aeruginosa exists in vivo biofilm influencing factors.