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目的初步探讨植物甾醇对人神经母细胞瘤SH-SY5Y细胞增殖和凋亡的影响。方法采用体外细胞培养的方法,用不同浓度的植物甾醇与细胞共同培养,倒置显微镜观察细胞形态,MTT法测定细胞存活率,流式细胞术测定细胞凋亡率。结果与对照组比较,从1μmol/L植物甾醇起即对细胞的存活率有明显影响,差异有统计学意义(P<0.01);植物甾醇浓度由低到高,随着浓度的增加,对细胞的形态影响越明显,突触变短,胞体萎缩甚至死亡;不同浓度的植物甾醇可引起细胞不同程度的早期凋亡。结论植物甾醇对体外培养的人神经母细胞瘤SHSY5Y细胞有潜在的毒性作用,能够抑制细胞增殖和引起细胞凋亡。
Objective To investigate the effects of phytosterols on the proliferation and apoptosis of human neuroblastoma SH-SY5Y cells. Methods In vitro cell culture was used to culture cells with different concentrations of phytosterols. Cell morphology was observed under inverted microscope. Cell viability was measured by MTT assay and apoptosis rate was measured by flow cytometry. Results Compared with the control group, the survival rate of cells was significantly affected by 1μmol / L phytosterol, the difference was statistically significant (P <0.01); phytosterol concentration from low to high, with the concentration increased, the cells The more obvious the influence of the morphology, the shorter the synapse, cell body atrophy or even death; different concentrations of plant sterols can cause different degrees of early apoptosis of cells. Conclusion Phytosterols have potential toxic effects on human neuroblastoma SHSY5Y cells cultured in vitro, which can inhibit cell proliferation and induce apoptosis.