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目的:探讨白藜芦醇(Resveratrol,Res)对体外培养的人肝癌细胞(HepG2)细胞周期分布的影响,为Res抗癌作用提供实验依据。方法:人肝癌细胞系HepG2细胞经不同浓度Res(0、25、50、100μmol/L)培养24、48、72 h。在相差显微镜下观察细胞的形态变化。将作用于Res的细胞经PBS洗涤,70%冷乙醇固定,PI染色,流式细胞仪检测(FCM)细胞周期时相分布以及细胞周期素蛋白依赖性激酶CDK2,周期素Cyclin E水平的变化。结果:白藜芦醇可抑制肝癌HepG2细胞增殖,其作用具有一定的时间和浓度依赖性,伴随细胞周期分布变化,G0/G1期比例下降,S期、G2/M期比例上调,且与对照组相比,实验组CDK2及Cyclin E表达增加。结论:白藜芦醇可能通过影响CDK2及Cyclin E的活性使HepG2细胞阻滞在S期,从而抑制肝癌HepG2细胞的增殖。
Objective: To investigate the effect of Resveratrol (Res) on the cell cycle distribution of human hepatoma cells (HepG2) cultured in vitro, and to provide experimental evidence for the anti-cancer effects of Res. METHODS: Human hepatocellular carcinoma cell line HepG2 cells were cultured for 24, 48, and 72 h at different concentrations of Res (0, 25, 50, and 100 μmol/L). The morphology of the cells was observed under a phase contrast microscope. The Res-treated cells were washed with PBS, fixed with 70% cold ethanol, stained with PI, and analyzed by flow cytometry (FCM), cell cycle distribution, and changes in cyclin-dependent kinase CDK2 and Cyclin E levels. RESULTS: Resveratrol could inhibit the proliferation of hepatocellular carcinoma HepG2 cells in a time- and concentration-dependent manner. With the change of cell cycle distribution, the proportion of G0/G1 phase decreased, and the proportion of S phase and G2/M phase increased. Compared with the group, the expression of CDK2 and Cyclin E in the experimental group increased. Conclusion: Resveratrol may inhibit HepG2 cell proliferation by inhibiting the activity of CDK2 and Cyclin E in HepG2 cells in S phase.