论文部分内容阅读
目的：研究大鼠实验性肝硬变晚期腹水形成与肿瘤坏死因子α（ＴＮＦα）及肠源性内毒素血症的关系，探讨肝硬变腹水形成的机理。方法：以大鼠为实验对象，分为正常对照组（ｎ＝８）、肝硬变对照组（ｎ＝１２）和肝硬变伴腹水组（ｎ＝１８）。实验组采用复合因子复制肝硬变动物模型。各组均测定腹主动脉血中ＴＮＦα和内毒素水平，肝硬变组还测定了腹水量。结果：随着肝硬变形成，大鼠血中ＴＮＦα水平增高，各组含量分别为９０８３±１０７１ｎｇ／ｍｌ和９５７７±１２７６ｎｇ／ｍｌ（肝硬变组和肝硬变伴腹水组）。相关分析表明，肝硬变组腹水量与血中ＴＮＦα成正相关（ｒ＝０８６，Ｐ＜００５），内毒素水平增高，并与ＴＮＦα的浓度成正相关（ｒ＝０７５，Ｐ＜００５）。结论：肝硬变腹水形成与血中ＴＮＦα升高有关，而ＴＮＦα的升高则源于肠源性内毒素血症的形成。
Objective: To study the relationship between the formation of ascites and TNF-α and enterogenous endotoxemia in rats with experimental cirrhosis and to investigate the mechanism of ascites formation in cirrhotic rats. Methods: The rats were divided into normal control group (n = 8), cirrhosis control group (n = 12) and cirrhosis with ascites group (n = 18). The experimental group used compound factor to replicate cirrhosis animal model. The levels of TNFα and endotoxin in abdominal aorta blood were determined in each group, and the amount of ascites was also measured in cirrhotic group. Results: With the development of cirrhosis, the levels of TNFα in the blood of rats increased, the levels in each group were 9083 ± 1071ng / ml and 9577 ± 1276ng / ml respectively (cirrhosis group and cirrhosis group With ascites group). Correlation analysis showed that the amount of ascites in cirrhotic patients was positively correlated with TNFα in blood (r = 0.86, P <005), and the level of endotoxin was increased, which was positively correlated with the concentration of TNFα (r = 0.7575, P <005). Conclusion: The formation of ascites in cirrhosis is related to the increase of TNFα in blood, while the increase of TNFα is due to the formation of enterogenous endotoxemia.