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目的用膜联蛋白Ｖ（Ａｎｎｅｘｉｎ Ｖ）法和碘化吡啶（ＰＩ）法两种流式细胞术定量检测肝细胞凋亡，作出应用评价，并用此方法探讨三七皂甙Ｒｇ１、 Ｒｂ１对急性肝损害的保护作用。方法用Ａｎｎｅｘｉｎ Ｖ法和ＰＩ法 检测脂多糖所致急性肝损害和 Ｒｇ１、 Ｒｂ１对肝损害的保护作用。用 ３Ｈ标记油酸法检测分泌型磷脂酶 Ａ２的活性。结果（ｌ）与ＰＩ染色法比， Ａｎｎｅｘｉｎ Ｖ法有更高的灵敏度和特异性，且仅用此法既能将正常细胞、凋亡细胞和坏死细胞区分开。（２）Ｒｇ１、 Ｒｂ１可明显降低脂多糖所致的大鼠急性肝损伤的凋亡率和坏死率（Ｐ＜ ０．０１），可明显降低ｓＰＬＡ２的活性（Ｐ＜ ０．０１）。结论（１）Ａｎｎｅｘｉｎ Ｖ 法是理想的定量检测凋亡细胞的方法。（２）Ｒｇ１、 Ｒｂ１可保护 ＬＰＳ所致的大鼠急性肝损害。
Objective To quantitatively detect apoptosis of hepatocytes by Annexin V and PI staining and to evaluate the application of this method in the study of the effects of notoginsenoside Rg1 and Rb1 on acute liver injury The protective effect. Methods Annexin V and PI were used to detect the acute liver injury induced by lipopolysaccharide and the protective effect of Rg1 and Rb1 on liver damage. The activity of secreted phospholipase A2 was detected by 3 H labeled oleic acid method. Results (1) Compared with the PI staining method, Annexin V method has higher sensitivity and specificity, and only this method can both normal cells, apoptotic cells and necrotic cells to distinguish. (2) Rg1 and Rb1 could significantly reduce the apoptosis rate and necrosis rate of acute hepatic injury induced by lipopolysaccharide in rats (P <0.01), and significantly reduce the activity of sPLA2 (P <0.01). Conclusions (1) Annexin V method is an ideal method for the quantitative detection of apoptotic cells. (2) Rg1, Rb1 can protect LPS-induced acute liver injury in rats.