对基于宽场随机荧光漂白的超分辨显微方法进行了研究。通过对获取的荧光漂白图像进行差分处理,获得大量稀疏的荧光分子图像,相应的荧光分子被一一区分并通过点扩散函数(PSF)数字化的方法计算出中心位置,从而重构超分辨显微图像。并对标记Alex 488普通荧光染料的人胚肾细胞进行了成像,和普通荧光显微镜相比,分辨率提升了约40%,表明该方法适用于常规荧光染料,并能突破衍射极限达到超分辨效果。该方法克服了常用的基于单分子精确定位技术需要对荧光分子进行反复地激活-猝灭,并难以适用于普通荧光染料的缺点。 A super-resolution microscopic method based on a wide-field random fluorescence bleaching was studied. A large number of sparse fluorescent molecular images are obtained by differential processing of the obtained fluorescence bleached image, the corresponding fluorescent molecules are respectively distinguished and digitized by a point spread function (PSF) to calculate the central position so as to reconstruct the super-resolution microscopic image. Compared with ordinary fluorescent microscope, the resolution of human embryo kidney cells labeled with Alex 488 common fluorescent dye was increased by about 40%, indicating that the method is suitable for conventional fluorescent dyes and can break through the diffraction limit to achieve super-resolution . This method overcomes the common shortcomings of single-molecule precise positioning technique that requires repeated activation-quenching of fluorescent molecules and is difficult to apply to common fluorescent dyes.