测定肿瘤细胞中的腺苷酸类,可了解肿瘤细胞能量异常代谢,同时在研究抗肿瘤药物的作用机理方面具有重要的意义。核苷酸类化合物具有很强的水溶性,用反相色谱法分离效果比离子交换色谱法更好,其优点是色谱柱易于平衡,结果的重复性好。本文在Schweinsberg测定红细胞方法的基础上,对梯度曲线及分析时间进行合理的调整和改进,并利用此方法测定了Hela细胞株和L_(78M)腹水白血病细胞腺苷酸类。 Determination of adenosine in tumor cells, can understand the abnormal energy metabolism of tumor cells, while in the study of the mechanism of action of anti-tumor drugs has important significance. Nucleotide compounds are highly water-soluble, and the separation by reversed-phase chromatography is better than ion-exchange chromatography. The advantage is that the column is easy to balance and the repeatability is good. Based on the method of Schweinsberg determination of erythrocytes, the gradient curve and analysis time were adjusted and improved rationally, and the adenylates of Hela cell line and L 78M ascitic leukemia cells were determined by this method.