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目的 探讨人类连接蛋白基因 2 6 (connexin2 6 ,Cx2 6 )的调控机理。方法 对 Cx2 6基因转译起始点上游的一个 DNase- 1超敏区片段 1.6 kb进行序列分析和 CAT报告基因分析。结果 Cx2 6 - 1.6 kb片段具有启动子功能。结论 Cx2 6 - 1.6 kb中含有 2个 GT盒 (分别位于 - 6 15 8bp和 - 6 2 13bp) ,1个TTAAAA盒位于 - 6 2 37bp,这是在人类 Cx2 6基因中新发现的第 2个启动区。
Objective To investigate the regulatory mechanism of connexin2 6 (Cx2 6) gene. Methods A DNA fragment of 1.6 kb upstream of the translation initiation site of Cx2 6 gene was analyzed by sequence analysis and CAT reporter gene analysis. Results Cx26 - 1.6 kb fragment has promoter function. Conclusions There are two GT boxes in Cx2 6 - 1.6 kb (- 6 15 8bp and - 6 2 13bp), one TTAAAA box in - 6 2 37bp, which is the second newly found in human Cx2 6 gene booting section.