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采用硅胶柱色谱、MPLC、制备与半制备HPLC等分离技术从蝉翼藤根的95%乙醇提取物中分离得到7个乙酰化的三萜皂苷类化合物。通过理化性质和MS,NMR等波谱数据鉴定其结构分别为蝉翼藤皂苷A(1),蝉翼藤皂苷B(2),3-O-β-Dglucopyranosyl presenegenin 28-O-β-D-xylopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→3)]-4-O-[(E)-3,4-dimethoxycinnamoyl]-β-D-fucopyranosyl ester(3),3-O-β-D-glucopyranosyl presenegenin 28-O-β-D-xylopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→3)]-4-O-[(E/Z)-3,4-dimethoxycinnamoyl]-β-D-fucopyranosyl ester(3/4),3-O-β-D-glucopyranosyl presenegenin 28-O-α-L-arabinopyranosyl-(1→3)-β-D-xylopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→2)-4-O-[(E)-3,4-dimethoxycinnamoyl]-β-D-fucopyranosyl ester(5),远志皂苷XLV(6),远志皂苷XLVI(7)。其中化合物5~7为首次从该属植物中分离得到,化合物3,3/4为首次从该种植物中分离获得。采用MTT法对所得化合物进行抗肿瘤活性评价,结果表明化合物2,3/4,5对肺癌细胞株LLC具有中等抑制作用,其IC50分别为41.10,38.17,48.92μmol·L-1;化合物2对乳腺癌细胞株MCF-7有中等抑制作用,其IC150为47.93μmol·L-。
Seven acetylated triterpene saponins were isolated from the 95% ethanolic extract of Cicadae cv. Wenggen by silica gel column chromatography, MPLC, preparative and semi-preparative HPLC. Their structures were identified by physico-chemical properties and MS, NMR and other spectroscopic data. The structures of the compounds were identified as follows: A (1), 3-O-β-Dglucopyranosyl presenegenin 28-O-β-D-xylopyranosyl - (1 → 4) -α-L-rhamnopyranosyl- (1 → 2) - [β-D-glucopyranosyl- (1 → 3)] - 4-0- [(E) -3,4-dimethoxycinnamoyl] -D-fucopyranosyl ester (3), 3-O-β-D-glucopyranosyl presenegenin 28-O-β-D-xylopyranosyl- (1- → 4) D-glucopyranosyl- (1 → 3)] - 4-O- [(E / Z) -3,4-dimethoxycinnamoyl] -β-D-fucopyranosyl ester (3/4) presenegenin 28-O-α-L-arabinopyranosyl- (1 → 3) -β-D-xylopyranosyl- (1 → 4) -α-L- rhamnopyranosyl- 3,4-dimethoxycinnamoyl] -β-D-fucopyranosyl ester (5), Polygalaceae saponin XLV (6), Polygalaceae saponin XLVI (7). Among them, compounds 5 to 7 were isolated from this genus for the first time. Compound 3, 3 and 4 were isolated from this plant for the first time. The anti-tumor activity of the compounds was evaluated by MTT assay. The results showed that the compounds 2,3,4,5 had a moderate inhibitory effect on LLC cell lines with IC50 of 41.10, 38.17 and 48.92μmol·L-1, respectively. Compound 2 The breast cancer cell line MCF-7 had a moderate inhibitory effect with an IC50 of 47.93 μmol·L-.