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目的:探讨肥大细胞(m ast cell,MC)在慢性肝病中的作用及乙型肝炎病毒(HBV)感染是否引起慢性肝病中MC数量增加。方法:本研究包括正常组(NL)8例、慢性肝炎组(CH)30例、肝硬化组(LC)43例、肝癌组(HCC)49例。采用甲苯胺蓝染色和免疫组织化学染色观察130例人肝组织中肥大细胞的密度和分布特征。另外,采用免疫组织化学染色定性检测各组HBsAg,HBcAg的表达。结果:各肝病组中(肝炎组、肝硬化组、肝癌组)肥大细胞密度比正常组显著增加(P<0.05);肝硬化组、肝癌组中MC密度均比慢性肝炎组显著增加(P<0.05);但肝硬化组与肝癌组之间差异无统计学意义(P>0.05)。MC分布以结缔组织区域多见。本组病例中肥大细胞密度与HBV感染无关。结论:肥大细胞可能参与慢性肝病发生发展过程并发挥重要作用,但其数量增加可能与HBV感染无直接关系。
Objective: To investigate the role of mast cells (MCs) in chronic liver disease (CHD) and whether hepatitis B virus (HBV) infection causes an increase in the number of MC in chronic liver disease. Methods: This study included 8 cases of normal group (NL), 30 cases of chronic hepatitis group (CH), 43 cases of liver cirrhosis group (LC) and 49 cases of liver cancer group (HCC). The density and distribution of mast cells in 130 human liver tissues were observed by toluidine blue staining and immunohistochemical staining. In addition, the immunohistochemical staining qualitative detection of HBsAg, HBcAg expression in each group. Results: The density of mast cells in the liver disease group (hepatitis group, cirrhosis group, liver cancer group) was significantly higher than that in the normal group (P <0.05). The MC density in the liver cirrhosis group and the liver cancer group was significantly higher than that in the chronic hepatitis group (P < 0.05). However, there was no significant difference between cirrhosis and hepatocellular carcinoma (P> 0.05). MC distribution is more common in connective tissue area. Mast cell density in this group of patients has nothing to do with HBV infection. Conclusion: Mast cells may play an important role in the development and progression of chronic liver disease, but their numbers may not be directly related to HBV infection.