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目的:脓毒症的发病率及死亡率居高不下,对脓毒症患者进行免疫功能调节一直是其重要治疗方法。大黄具有泻下,抑制内毒素移位及减轻炎症反应功能;乌司他丁(UTⅠ)具有调节、增强免疫功能,此次分析探讨大黄联合UTⅠ对脓毒症大鼠免疫功能的影响。方法:将120只成年SD大鼠(体重210~250g)随机分成正常对照组(Ⅰ组)、阑尾结扎穿孔(CLP)模型组(Ⅱ组)、CLP后大黄联合UTⅠ治疗组(Ⅲ组),每组40只。Ⅰ组开腹找到盲肠后立即关腹,Ⅱ组按文献报道方法进行处理,使大鼠腹腔感染继发脓毒症,Ⅲ组在Ⅱ组基础上联合给予大黄及UTⅠ治疗(12h给药一次),每组分别在CLP后6h、12h、24h、36h抽取心脏血,每个时间点10只大鼠,分析比较各组大鼠T淋巴细胞亚群。
OBJECTIVE: The high morbidity and mortality of sepsis has been an important treatment for immune function in sepsis patients. Rhubarb has diarrhea, inhibit endotoxin translocation and reduce the inflammatory response; Ulinastatin (UT Ⅰ) with regulation, enhance immune function, the analysis of rhubarb combined with UT Ⅰ on immune function in septic rats. Methods: 120 adult SD rats weighing 210-250 g were randomly divided into normal control group (group Ⅰ), CLP model group (group Ⅱ), rhubarb combined UT Ⅰ group (group Ⅲ) 40 per group Group Ⅰ was closed immediately after finding the cecum, group Ⅱ was treated according to the method reported in the literature, secondary sepsis was induced by intraperitoneal infection in group Ⅲ, rhubarb and UTⅠ were given to group Ⅲ on the basis of group Ⅱ (once for 12 hours) The blood of each group was drawn at 6h, 12h, 24h and 36h after CLP, respectively, and 10 rats at each time point were used to analyze and compare the T lymphocyte subsets of rats in each group.