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目的:建立野木瓜中木犀草素的含量测定方法。方法:Dikma Diamonsil C18色谱柱(4.6 mm×250 mm,5μm),流动相乙睛-0.1%磷酸溶液(26∶74),流速1 mL.min-1,检测波长350 nm,柱温室温。结果:线性范围0.067 75～2.168μg(r=0.999 9),平均回收率为99.25%,RSD 1.65%,精密度RSD 2.22%,重复性RSD 1.24%,稳定性RSD 2.58%。结论:方法稳定、可靠,可作为该药材的质量控制方法。
Objective: To establish the determination method of luteolin in wild papaya. METHODS: Dikma Diamonsil C18 column (4.6 mm × 250 mm, 5 μm) was used. The mobile phase was acetonitrile-0.1% phosphoric acid solution (26:74) and the flow rate was 1 mL.min-1. The detection wavelength was 350 nm and the column temperature was at room temperature. Results: The linear range was 0.067 75-2.168 μg (r = 0.999 9). The average recovery was 99.25%, RSD 1.65%, RSD precision 2.22%, RSD 1.24% and RSD 2.58%. Conclusion: The method is stable and reliable and can be used as quality control method of this medicine.