论文部分内容阅读
目的:本研究旨在说明全天麻胶囊及天麻素联合卡马西平应用于抗癫痫治疗后,对于卡马西平抗癫痫作用的影响及作用机制。方法:通过戊四唑诱导的癫痫小鼠模型,采用癫痫模型发作等级评分,对不同给药组癫痫模型小鼠进行评分,以判断联合给药后卡马西平抗癫痫作用。通过普通病理学、RT-PCR研究全天麻胶囊及天麻素联合卡马西平对癫痫小鼠脑部海马体神经元及多药耐药相关蛋白基因(mrp1)的影响。结果:全天麻及天麻素联合卡马西平能够显著提高卡马西平的抗癫痫作用,全天麻胶囊可改善海马CA3区神经元损伤,而天麻素及卡马西平则无该类作用。全天麻胶囊可逆转因多次给药引起的mrp1基因的高表达,天麻素则会在多次给药后诱导mrp1基因高表达。结论:全天麻胶囊可提高卡马西平的抗癫痫小鼠作用,与减轻海马神经元损伤及降低mrp1基因高表达相关。
OBJECTIVE: This study was designed to demonstrate the effect and mechanism of action of all-Tianma capsule and gastrodin combined with carbamazepine on the anti-epileptic effect of carbamazepine. Methods: Epileptic mouse model induced by pentylenetetrazole was used to evaluate the antiepileptic effect of carbamazepine after the combined administration of epileptic seizure mice in different administration groups. The effects of Quantiandi capsule and gastrodin combined with carbamazepine on the hippocampal neurons and multidrug resistance-associated protein gene (mrp1) in brain of epileptic mice were studied by routine pathology and RT-PCR. Results: All Tianma and gastrodin combined with carbamazepine could significantly enhance the antiepileptic effect of carbamazepine. All Tianma capsule could improve the neuronal damage in CA3 area of hippocampus, whereas gastrodin and carbamazepine had no such effect. All-Tianma capsule can reverse the high expression of mrp1 gene caused by multiple administrations, while gastrodin induces the high expression of mrp1 gene after multiple administrations. Conclusion: All Tianma capsule can improve the anti-epileptic mice induced by carbamazepine, which is related to the decrease of hippocampal neuron injury and the decrease of mrp1 gene expression.