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目的：探讨ｎｍ２３－Ｈ１蛋白表达与大肠癌肝转移及预后的关系。方法：对１０１例大肠癌存档石蜡块进行重新切片，采用ｎｍ２３－Ｈ１单克隆抗体进行免疫组化染色（ＬＳＡＢ法）。结果：ｎｍ２３－Ｈ１蛋白表达与年龄、性别、肿瘤大小、部位、组织类型、浆膜侵犯无关；与Ｄｕｋｅｓ分期、淋巴结转移有关；手术时有肝转移组较无肝转移组低，手术后有肝转移复发组较无肝转移复发组低（Ｐ＜００１）。Ｃｏｘ模型分析显示ｎｍ２３－Ｈ１是大肠癌预后的一个保护性指标。结论：ｎｍ２３－Ｈ１基因对大肠癌肝转移和预后具有重要作用。ＬＳＡＢ法检测大肠癌组织中ｎｍ２３－Ｈ１蛋白表达可能是预测大肠癌肝转移及预后的生物学指标之
Objective: To investigate the relationship between nm23-H1 protein expression and hepatic metastasis and prognosis of colorectal cancer. METHODS: 101 cases of colorectal cancer paraffin blocks were re-sectioned and immunohistochemically stained with the nm23-H1 monoclonal antibody (LSAB method). RESULTS: The expression of nm23-H1 protein was not related to age, sex, tumor size, location, histological type, and serosal invasion. It was related to Dukes stage and lymph node metastasis. Hepatic metastasis group had lower liver metastasis group than hepatic metastasis group after surgery. Metastasis recurrence group was lower than that without liver metastasis and recurrence group (P<001). Cox model analysis showed that nm23-H1 is a protective index for the prognosis of colorectal cancer. Conclusion: The nm23-H1 gene plays an important role in the liver metastasis and prognosis of colorectal cancer. Detection of nm23-H1 protein expression in colorectal cancer by LSAB may be a biological indicator for predicting hepatic metastasis and prognosis of colorectal cancer