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目的了解无锡市门诊就诊腹泻病例中病毒感染情况。方法对2013-2015年无锡市腹泻监测哨点医院门诊腹泻病例的粪便标本,采用实时荧光PCR方法对收集的样本进行轮状病毒(Rotavirus,RV)、诺如病毒(NV)、肠道腺病毒(EADV)、札如病毒(SPAV)和星状病毒(ASTV)的核酸检测,在中国疾病预防控制信息系统“突发公共卫生事件管理信息系统”中导出肠道感染性疾病突发公共卫生事件的相关信息和数据进行分析。结果 2013-2015年无锡市腹泻病人标本检测病毒总阳性率为17.61%。NV和RV的阳性率最高,分别为11.68%和4.02%,混合感染率1.52%。NV中GⅡ型占88.14%,GⅠ型占11.86%。NV和RV感染有明显的季节特征,以寒冷季节高发。NV在12-17岁年龄组(29.03%)和学生(14.67%)人群中阳性率最高;RV在0-5岁年龄组和学龄前儿童(均为9.00%)的阳性率最高。感染性腹泻暴发疫情中诺如病毒感染的构成比高(58.50%)。结论 NV和RV是无锡市2013-2015年病毒性腹泻的优势病原体。诺如病毒所致暴发疫情的发生数和构成比高,应重点加强诺如病毒的监测。
Objective To understand the prevalence of viral infections in outpatient diarrhea cases in Wuxi City. Methods The stool samples from diarrhea clinics of sentinel hospital of Wuxi diarrhea monitoring center from 2013 to 2015 were collected. Real-time fluorescence PCR was used to detect rotavirus (RV), norovirus (NV), enteric adenovirus (EADV), Sapporo virus (SPAV) and Astrovirus (ASTV) nucleic acid detection in China’s disease prevention and control information system “public health emergency management information system ” out of the public outbreak of infectious diseases of the public Health events related information and data analysis. Results The total positive rate of virus detection in diarrhea patients in Wuxi from 2013 to 2015 was 17.61%. The positive rates of NV and RV were the highest, which were 11.68% and 4.02% respectively, and the mixed infection rate was 1.52%. G Ⅱ in NV accounted for 88.14%, G Ⅰ accounted for 11.86%. NV and RV infections have obvious seasonal characteristics, high in the cold season. The positive rate of NV was the highest in 12-17 age group (29.03%) and students (14.67%). The positive rate of RV was the highest in 0-5 age group and preschool children (9.00%). Infectious diarrhea outbreaks of norovirus infection constitute a high proportion (58.50%). Conclusion NV and RV are the predominant pathogens of viral diarrhea in Wuxi in 2013-2015. Novo virus outbreaks caused by the number and the composition ratio of the high, should focus on strengthening the monitoring of Norovirus.