利用冰冻切片制作电镜标本 ,由于冰晶的大量出现无法进行超微结构的识别 ,故很少有人涉足。在免疫电镜及酶细胞电镜等研究中 ,需要定位后进行电镜标本制作 ,为了避免冰晶的出现 ,只能使用非常烦琐的振动切片机切片。若进行系统的光镜、电镜观察 ,就更加困难。为此我们经过反复 The use of frozen sections of electron microscopy specimens, due to the large number of ice crystals can not be identified ultrastructure, so few people involved. In immunoelectron microscopy and enzyme cell electron microscopy and other studies, the need for positioning electron microscopy specimens, in order to avoid the emergence of ice crystals, can only be used very cumbersome vibrating microtome section. If the system of light microscopy, electron microscopy, even more difficult. To this end we have repeated