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为进一步确定膳食多不饱和脂肪酸的添加提供依据,本文选取与人类基因高度相似的模式生物斑马鱼为研究对象,采用气质联用法分析斑马鱼不同生长时间(四月龄,六月龄和七月龄)和不同生理部位(大脑、心脏、肾脏、尾鳍、鱼腹肉以及整鱼)中的脂肪酸的含量与组成。分析结果表明:在斑马鱼中共鉴定出25种脂肪酸;除斑马鱼整鱼外,鱼腹肉中的脂肪酸种类最多,为20种;其它器官中的脂肪酸皆为10余种。在所有的组织中,棕榈酸(C_(16:0)),硬脂酸(C_(18:0))和油酸(C_(18:1))都是主要的脂肪酸,约占各组织器官总脂肪酸的50%以上;在整鱼与鱼腹肉中,不饱和脂肪酸的含量高于饱和脂肪酸,而在大脑、心脏、肾脏和尾鳍中,情况相反。对成熟斑马鱼大脑的不同部位(端脑、视顶盖和小脑)中的脂肪酸组成研究表明,各部分的脂肪酸的种类无显著性差异。
To further determine the dietary polyunsaturated fatty acids to provide a basis for the selection of human genes highly similar to the model of biological zebrafish for the study, the use of GC-MS analysis of zebrafish growth time (four months, six months of age and July Age) and fatty acid content and composition in different physiological sites (brain, heart, kidney, caudal fin, fish belly and whole fish). The results showed that: 25 kinds of fatty acids were identified in zebrafish; except zebrafish whole fish, the flesh belly meat in the most fatty species, 20 species; other organs are all more than 10 kinds of fatty acids. Palmitic acid (C_ (16: 0)), stearic acid (C_ (18: 0)) and oleic acid (C_ (18: 1)) are the main fatty acids in all the tissues, More than 50% of the total fatty acids; in whole fish and fish belly meat, the content of unsaturated fatty acids is higher than that of saturated fatty acids, whereas in the brain, heart, kidney and caudal fin, the opposite is true. Studies on the fatty acid composition of different parts of the brain of mature zebrafish (telencephalon, optic cap, and cerebellum) showed no significant difference in the types of fatty acids in each part.