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应用不连续蔗糖／甘油密度梯度超速离心的方法，从培养细胞中纯化甲肝病毒（Ｈｅ－ｐａｔｉｔｉｓＡＶｉｒｕｓ，ＨＡＶ）。纯化的ＨＡＶ抗原经ＳＤＳ－ＰＡＧＥ电泳分析，获得三条带：ＶＰ１（３４０００），ＶＰ２（３００００）和ＶＰ３（２６０００）。用纯化的ＨＡＶ抗原免疫Ｂａｌｂ／ｃ小鼠，将已免疫小鼠的脾细胞与ＳＰ２／０细胞融合，获得６株分泌抗ＨＡＶ单克隆抗体的杂交瘤。它们的培养上清和腹水的抗体滴度分别是５０～１０００和１０００～４０００。Ｉｇ亚类５株为ＩｇＧ１，１株为ＩｇＧ２ａ。采用间接的ＥＬＩＳＡ法分析ＨＡＶ抗原的抗原位点，结果表明ＨＡＶ抗原至少存在４个抗原位点，两个存在于ＶＰ１蛋白带，另两个分别存在于ＶＰ２和ＶＰ３蛋白带
Hepatoma virus (HAV) was purified from cultured cells using a method of discontinuous sucrose / glycerol density gradient ultracentrifugation. Purified HAV antigens were analyzed by SDS-PAGE and three bands were obtained: VP1 (34000), VP2 (30000) and VP3 (26000). Balb / c mice were immunized with the purified HAV antigen and the spleen cells of the immunized mice were fused with SP2 / 0 cells to obtain 6 hybridomas secreting anti-HAV monoclonal antibodies. Their culture supernatants and ascites antibody titers were 50 to 1000 and 1000 to 4000, respectively. Five strains of Ig subclasses were IgG1 and one strain was IgG2a. Analysis of the antigenic site of the HAV antigen using an indirect ELISA showed that there were at least 4 antigenic sites in the HAV antigen, two in the VP1 protein band and the other two in the VP2 and VP3 protein bands