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目的研制胶体金-葡萄球菌A蛋白试剂盒用于检测日本血吸虫抗体。方法通过不同筛目尼龙绢和低频超声等方法,制备、纯化日本血吸虫可溶性虫卵抗原,研制胶体金-葡萄球菌A蛋白试剂盒。用该试剂盒检测人血清中日本血吸虫特异性抗体,并用ELISA方法进行平行比较。结果胶体金-葡萄球菌A蛋白试剂盒检测慢性血吸虫病的敏感性为93%,检测急性血吸虫病的敏感性为100%,检测健康人血清的特异性为96%,检测肺吸虫病的交叉反应为40%,对其他寄生虫和乙肝未见有交叉反应。与ELISA检测结果比较,两种方法具有良好的一致性。结论胶体金-葡萄球菌A蛋白试剂盒具有快速、简便、不需要仪器、敏感性高和特异性强等优点,可广泛用于血吸虫病流行区现场查病。
Objective To develop a colloidal gold-staphylococcal protein A kit for the detection of Schistosoma japonicum antibody. Methods The soluble egg antigen of Schistosoma japonicum was prepared and purified by different methods, such as nylon mesh and low-frequency ultrasound, and the colloidal gold-staphylococcal protein A kit was developed. The kit was used to detect specific antibodies against Schistosoma japonicum in human serum and compared by ELISA. Results The sensitivity of colloidal gold-staphylococcal protein A kit for detection of chronic schistosomiasis was 93%, the sensitivity for detecting acute schistosomiasis was 100%, the specificity for detecting serum was 96%, and the cross-reaction of paragonimiasis 40%, no cross reaction to other parasites and hepatitis B. Compared with ELISA test results, the two methods have good consistency. Conclusion The colloidal gold-staphylococcal protein A kit has the advantages of fast, simple, no need of instruments, high sensitivity and specificity. It can be widely used in the field of schistosomiasis endemic diseases.