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目的:研究睫状神经营养因子(ciliary neurontrophic factor,CNTF)对糖尿病早期大鼠视网膜神经组织的保护作用。方法:选择40只健康成年雄性SD大鼠(250g-280g)一次性腹腔注射链脲佐菌素(Streptozotin,STZ)60mg/kg诱发糖尿病模型(DM),将DM大鼠随机分组为DM+CNTF组和DM+BSS组。DM+CNTF组给予玻璃体腔内注射CNTF(1.0μg/2μL),DM+BSS组注射BSS液(2μL)。分别观测0、4、8、12wk两组大鼠体质量和血糖变化,于4wk和12wk进行原位细胞调亡(TUNEL法)的检测及视网膜神经组织超微结构的观察。结果:DM+CNTF组大鼠的血糖和体质量与DM+BSS组比较无显著性差异(P>0.05)。12wk时TUNEL检测DM+CNTF组大鼠神经节细胞凋亡与DM+BSS组相比减少(P<0.05)。透射电镜下观察发现从4wk起两组大鼠视网膜神经组织出现细胞凋亡的改变,经CNTF治疗细胞凋亡改变有所减轻,表现为外节膜盘间隙减小,感光细胞水肿减轻及核染色质浓集减轻等。结论:CNTF对DM+CNTF组和DM+BSS组大鼠的体重及血糖无明显影响。CNTF治疗组结果显示对本实验糖尿病大鼠视网膜神经节细胞及感光细胞有一定保护作用。
Objective: To study the protective effect of ciliary neurontrophic factor (CNTF) on the retinal nerve tissue in early diabetic rats. Methods: Forty healthy adult male Sprague-Dawley rats (250g-280g) were given a single intraperitoneal injection of 60mg / kg streptozotin (STZ) to induce diabetes mellitus (DM). DM rats were randomly divided into DM + CNTF Group and DM + BSS group. The DM + CNTF group was given intravitreal injection of CNTF (1.0μg / 2μL) and the DM + BSS group was injected with BSS solution (2μL). The changes of body weight and blood glucose of rats in 0, 4, 8, and 12 weeks were observed. TUNEL assay and ultrastructure of retina were observed at 4wk and 12wk respectively. Results: The blood glucose and body weight of rats in DM + CNTF group had no significant difference compared with DM + BSS group (P> 0.05). At 12wk, the apoptosis of ganglion cells in DM + CNTF group was lower than that in DM + BSS group (P <0.05). Transmission electron microscopy showed that retinal ganglion cells in both groups changed from 4wk after apoptosis, and the change of apoptotic cells was alleviated by CNTF treatment, which showed that the outer membrane disc space was reduced, the photoreceptor cell edema and nuclear staining were reduced Mass concentration reduction and so on. Conclusion: CNTF has no effect on body weight and blood glucose in DM + CNTF group and DM + BSS group. CNTF treatment group showed that the experimental retinal ganglion cells and photoreceptor cells in diabetic rats have a protective effect.